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Drosophila sperm development and intercellular cytoplasm sharing through ring canals do not require an intact fusome.
Kaufman, Ronit S; Price, Kari L; Mannix, Katelynn M; Ayers, Kathleen M; Hudson, Andrew M; Cooley, Lynn.
Afiliação
  • Kaufman RS; Department of Genetics, Yale University School of Medicine, New Haven, CT 06520, USA.
  • Price KL; Department of Genetics, Yale University School of Medicine, New Haven, CT 06520, USA.
  • Mannix KM; Department of Genetics, Yale University School of Medicine, New Haven, CT 06520, USA.
  • Ayers KM; Department of Genetics, Yale University School of Medicine, New Haven, CT 06520, USA.
  • Hudson AM; Department of Genetics, Yale University School of Medicine, New Haven, CT 06520, USA.
  • Cooley L; Department of Genetics, Yale University School of Medicine, New Haven, CT 06520, USA lynn.cooley@yale.edu.
Development ; 147(22)2020 11 15.
Article em En | MEDLINE | ID: mdl-33033119
Animal germ cells communicate directly with each other during gametogenesis through intercellular bridges, often called ring canals (RCs), that form as a consequence of incomplete cytokinesis during cell division. Developing germ cells in Drosophila have an additional specialized organelle connecting the cells called the fusome. Ring canals and the fusome are required for fertility in Drosophila females, but little is known about their roles during spermatogenesis. With live imaging, we directly observe the intercellular movement of GFP and a subset of endogenous proteins through RCs during spermatogenesis, from two-cell diploid spermatogonia to clusters of 64 post-meiotic haploid spermatids, demonstrating that RCs are stable and open to intercellular traffic throughout spermatogenesis. Disruption of the fusome, a large cytoplasmic structure that extends through RCs and is important during oogenesis, had no effect on spermatogenesis or male fertility under normal conditions. Our results reveal that male germline RCs allow the sharing of cytoplasmic information that might play a role in quality control surveillance during sperm development.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermátides / Espermatogênese / Espermatogônias / Citoplasma / Meiose Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermátides / Espermatogênese / Espermatogônias / Citoplasma / Meiose Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article