Construction and characterization of the PGN_0296 mutant of Porphyromonas gingivalis.
J Oral Biosci
; 62(4): 322-326, 2020 12.
Article
em En
| MEDLINE
| ID: mdl-33038516
ABSTRACT
The periodontal pathogen Porphyromonas gingivalis produces gingipains (Kgp, RgpA, and RgpB), cysteine proteases involved in the organism's virulence, and pigmentation. We previously showed that deletion of the PGN_0297 and PGN_0300 genes reduced the proteolytic activity of gingipains. The role of the PGN_0296 gene, consisting of an operon with the PGN_0297 and PGN_0300 genes, is unclear. Herein, we examined the effect of PGN_0296 gene deletion on the proteolytic activity. Although the proteolytic activity of the gingipains did not decrease in the culture supernatant of a PGN_0296 gene deletion mutant (ΔPGN_0296), the growth was delayed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Porphyromonas gingivalis
/
Adesinas Bacterianas
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article