Platinum nanozyme-encapsulated poly(amidoamine) dendrimer for voltammetric immunoassay of pro-gastrin-releasing peptide.
Anal Chim Acta
; 1134: 106-114, 2020 Oct 16.
Article
em En
| MEDLINE
| ID: mdl-33059856
An innovative electrochemical immunosensing platform was designed for the sensitive monitoring of lung cancer biomarker (pro-gastrin-releasing peptide; ProGRP) by using platinum nanoparticles encapsulated inside dendrimers (PtDEN) as enzymatic mimics for the signal amplification. PtDEN nanocomposites were prepared through a simple chemical reduction method with the assistance of NaBH4. Thereafter, PtDEN-labeled anti-ProGRP secondary antibody was launched for the detection of target analyte with a sandwich-type assay format on anti-ProGRP capture antibody-modified screen-printed carbon electrode. Accompanying formation of immunocomplex, the labeled PtDENs electrochemically oxidized 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide to produce a well-defined voltammetric signal within the applied potentials. Thanks to the high-efficient catalytic efficiency of platinum nanoparticles and high-loading ability of dendrimer, improved analytical features were acquired with PtDENs relative to platinum nanoparticles alone. Using PtDENs labeling strategy, the properties and factors influencing the analytical performance of electrochemical immunosensor were studied in detail. The strong bioconjugation of antibodies with the PtDENs caused a good repeatability and intermediate precision down to 7.64%. Under optimum conditions, the electrochemical immunosensor exhibited a dynamic linear range of 0.001-10 ng mL-1 ProGRP with a detection limit of 0.86 pg mL-1. Good selectivity and relatively long-term stability (>6 months) were achieved for target ProGRP. Significantly, the acceptable accuracy was gotten for analysis of ProGRP in human serum specimens referring to commercially available human ProGRP enzyme-linked immunosorbent assay (ELISA) method.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Peptídeo Liberador de Gastrina
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Dendrímeros
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Nanopartículas Metálicas
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Neoplasias Pulmonares
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article