Development of redox potential-driven fermentation process for recombinant protein expression.
Biotechnol Lett
; 43(1): 99-103, 2021 Jan.
Article
em En
| MEDLINE
| ID: mdl-33064228
ABSTRACT
OBJECTIVES:
A redox potential-driven fermentation, maintaining dissolved oxygen at a prescribed level while simultaneously monitoring the changes of fermentation redox potential, was developed to guide the cultivation progress of recombinant protein expression.RESULTS:
A recombinant E. coli harboring prolinase-expressing plasmid (pKK-PepR2) was cultivated using the developed process. Two distinct ORP valleys were noticeable based on recorded profile. The first ORP valley is equivalent to the timing for the addition of inducing agent, and the second ORP valley serves to guide the timing for cell harvesting. The final prolinase activity is 0.172 µmol/mg/min as compared to that of 0.154 µmol/mg/min where the optical density was employed to guide the timing of inducer addition and an empirically determined length of the cultivation.CONCLUSION:
The developed process can be further modified to become an automatic operation.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxirredução
/
Biotecnologia
/
Proteínas Recombinantes
/
Fermentação
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article