Monoclonal antibody against a sperm antigen Mr 95,000 inhibits attachment of human spermatozoa to the zona pellucida.
J Reprod Immunol
; 11(3): 157-66, 1987 Jul.
Article
em En
| MEDLINE
| ID: mdl-3309284
ABSTRACT
A murine monoclonal antibody raised against hamster spermatozoa was found to cross-react with human spermatozoa. By immunofluorescence, the antigen was visualized over the equatorial segment of human sperm heads. In the presence of antibody, sperm binding to the zona pellucida of salt-stored human oocytes was significantly inhibited (P less than or equal to 0.005) compared with other antibodies or control preparations. Using SDS-PAGE of whole spermatozoa and membrane preparations followed by Western blot analysis, the antigen was identified as a determinant with a relative molecular weight of 95,000.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Óvulo
/
Interações Espermatozoide-Óvulo
/
Espermatozoides
/
Zona Pelúcida
/
Anticorpos Monoclonais
/
Antígenos
Limite:
Female
/
Humans
/
Male
Idioma:
En
Ano de publicação:
1987
Tipo de documento:
Article