Extracellular Multi-Unit Recording from the Olfactory Nerve of Teleosts.
J Vis Exp
; (164)2020 10 06.
Article
em En
| MEDLINE
| ID: mdl-33104073
ABSTRACT
Recent studies have shown that ocean acidification affects olfactory-driven behavior in fish. This may be due in part to a reduction in olfactory sensitivity in high PCO2/low pH water. To assess the effects of ocean acidification, or olfactory sensitivity in marine fish in general, we propose that extracellular multi-unit recording from the olfactory nerve is the method of choice. Although invasive, it is sensitive, robust, reproducible and independent of external salinity (unlike the electro-olfactogram [EOG], for example). Furthermore, it records a primary sensory input into the CNS, prior to any central processing. We show that this method can show a reduction in olfactory sensitivity that is both temporary and odorant-dependent, using a range of amino acids to construct concentration-response curves and calculate the thresholds of detection.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nervo Olfatório
/
Espaço Extracelular
/
Peixes
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article