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Severe Acute Respiratory Syndrome Coronavirus-2 Spike Protein Nanogel as a Pro-Antigen Strategy with Enhanced Protective Immune Responses.
Chen, Long; Liu, Bo; Sun, Peng; Wang, Wenjun; Luo, Shiqiang; Zhang, Wenyuan; Yang, Yuanfan; Wang, Zihao; Lin, Jian; Chen, Peng R.
Afiliação
  • Chen L; Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
  • Liu B; Department of Microorganism Engineering, Beijing Institute of Biotechnology, Beijing, 100071, China.
  • Sun P; Department of Microorganism Engineering, Beijing Institute of Biotechnology, Beijing, 100071, China.
  • Wang W; Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, College of Life Sciences, University of the Chinese Academy of Sciences, Beijing, 100101, China.
  • Luo S; Department of Microorganism Engineering, Beijing Institute of Biotechnology, Beijing, 100071, China.
  • Zhang W; Institute of Physical Science and Information, Anhui University, Hefei, 230601, China.
  • Yang Y; Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
  • Wang Z; Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
  • Lin J; Beijing Institute of Pharmacology and Toxicology, Beijing, 100850, China.
  • Chen PR; Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
Small ; 16(46): e2004237, 2020 11.
Article em En | MEDLINE | ID: mdl-33107685
ABSTRACT
Prevention and intervention methods are urgently needed to curb the global pandemic of coronavirus disease-19 caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Herein, a general pro-antigen strategy for subunit vaccine development based on the reversibly formulated receptor binding domain of SARS-CoV-2 spike protein (S-RBD) is reported. Since the poor lymph node targeting and uptake of S-RBD by antigen-presenting cells prevent effective immune responses, S-RBD protein is formulated into a reversible nanogel (S-RBD-NG), which serves as a pro-antigen with enhanced lymph node targeting and dendritic cell and macrophage accumulation. Synchronized release of S-RBD monomers from the internalized S-RBD-NG pro-antigen triggers more potent immune responses in vivo. In addition, by optimizing the adjuvant used, the potency of S-RBD-NG is further improved, which may provide a generally applicable, safer, and more effective strategy for subunit vaccine development against SARS-CoV-2 as well as other viruses.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glicoproteína da Espícula de Coronavírus / Nanogéis / SARS-CoV-2 / COVID-19 / Imunidade / Antígenos Virais Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glicoproteína da Espícula de Coronavírus / Nanogéis / SARS-CoV-2 / COVID-19 / Imunidade / Antígenos Virais Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article