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A highly sensitive biosensor with a single-copy evolved sensing cassette for chlorpyrifos pesticide detection.
Chouichit, Pakwilai; Whangsuk, Wirongrong; Sallabhan, Ratiboot; Mongkolsuk, Skorn; Loprasert, Suvit.
Afiliação
  • Chouichit P; Applied Biological Sciences Program, Chulabhorn Graduate Institute, Chulabhorn Royal Academy, Bangkok 10210, Thailand.
  • Whangsuk W; Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok 10210, Thailand.
  • Sallabhan R; Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok 10210, Thailand.
  • Mongkolsuk S; Center of Excellence on Environmental Health and Toxicology, Ministry of Education, Bangkok 10400, Thailand.
  • Loprasert S; Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok 10210, Thailand.
Microbiology (Reading) ; 166(11): 1019-1024, 2020 11.
Article em En | MEDLINE | ID: mdl-33108264
A formylglycine-generating enzyme (FGE)-sulfatase-based whole-cell biosensor was genetically improved into a single-copy system by integrating the Sinorhizobium meliloti transcriptional activator ChpR and the chpA promoter-FGE-sulfatase fusion into the Escherichia coli chromosome. The sensitivity was further enhanced through a random mutagenesis of the chpR. The new integrated biosensor offered both a lower detection limit [5 nM chlorpyrifos (CPF)] and fluorescence background. The ready-to-use kit was developed using silica gel for on-field detection. The biosensor kit was stable for 20 days when stored at 4 °C. Moreover, a 1-(1-naphthylmethyl)-piperazine (NMP) efflux pump inhibitor can improve the sensitivity by 57 %.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Praguicidas / Técnicas Biossensoriais / Clorpirifos Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Praguicidas / Técnicas Biossensoriais / Clorpirifos Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2020 Tipo de documento: Article