Single-Cell Isolation from Regenerating Murine Muscles for RNA-Sequencing Analysis.
STAR Protoc
; 1(2): 100051, 2020 09 18.
Article
em En
| MEDLINE
| ID: mdl-33111097
ABSTRACT
Single-cell RNA sequencing (scRNA-seq) is a powerful technique for deconvoluting and clustering thousands of otherwise intermingled cells based on their gene expression. Here, we present a complete protocol for the unbiased evaluation of regenerating murine skeletal muscle using scRNA-seq. The skeletal muscle is unique in its cellular composition as being primarily multinucleated muscle cells (myofibers). This protocol focuses on isolating mononuclear cells from muscle for subsequent scRNA-seq analysis and can be modified to assess cell populations in other tissues of interest. For complete details on the use and execution of this protocol, please refer to Liu et al. (2015) and Oprescu et al. (2020).
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regeneração
/
Análise de Célula Única
/
RNA-Seq
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Músculos
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article