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Comparative analysis of genome-wide DNA methylation identifies patterns that associate with conserved transcriptional programs in osteosarcoma.
Mills, Lauren J; Scott, Milcah C; Shah, Pankti; Cunanan, Anne R; Deshpande, Archana; Auch, Benjamin; Curtin, Bridget; Beckman, Kenneth B; Spector, Logan G; Sarver, Aaron L; Subramanian, Subbaya; Richmond, Todd A; Modiano, Jaime F.
Afiliação
  • Mills LJ; Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA; Childhood Cancer Genomics Group, Department of Pediatric, School of Medicine, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: ljmills@umn.edu.
  • Scott MC; Department of Microbiology and Immunology, Center for Immunology, School of Medicine, University of Minnesota, Minneapolis, MN 55455, USA.
  • Shah P; Roche Sequencing Solution, Pleasanton, CA, USA.
  • Cunanan AR; Roche Sequencing Solution, Pleasanton, CA, USA.
  • Deshpande A; University of Minnesota Genomics Center, Minneapolis, MN 55455, USA.
  • Auch B; University of Minnesota Genomics Center, Minneapolis, MN 55455, USA.
  • Curtin B; Department of Surgery, University of Minnesota, Minneapolis, MN 55455, USA.
  • Beckman KB; University of Minnesota Genomics Center, Minneapolis, MN 55455, USA.
  • Spector LG; Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA; Childhood Cancer Genomics Group, Department of Pediatric, School of Medicine, University of Minnesota, Minneapolis, MN 55455, USA.
  • Sarver AL; Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA; Animal Cancer Care and Research Program, University of Minnesota, St. Paul 55108, USA; Institute of Health Informatics, University of Minnesota, Minneapolis, MN 55455, USA.
  • Subramanian S; Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA; Department of Surgery, University of Minnesota, Minneapolis, MN 55455, USA.
  • Richmond TA; Roche Sequencing Solution, Pleasanton, CA, USA.
  • Modiano JF; Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA; Animal Cancer Care and Research Program, University of Minnesota, St. Paul 55108, USA; Institute of Health Informatics, University of Minnesota, Minneapolis, MN 55455, USA; Department of Veterinary Clinical Sciences, College
Bone ; 158: 115716, 2022 05.
Article em En | MEDLINE | ID: mdl-33127576
ABSTRACT
Osteosarcoma is an aggressive tumor of the bone that primarily affects young adults and adolescents. Osteosarcoma is characterized by genomic chaos and heterogeneity. While inactivation of tumor protein p53 (TP53) is nearly universal other high frequency mutations or structural variations have not been identified. Despite this genomic heterogeneity, key conserved transcriptional programs associated with survival have been identified across human, canine and induced murine osteosarcoma. The epigenomic landscape, including DNA methylation, plays a key role in establishing transcriptional programs in all cell types. The role of epigenetic dysregulation has been studied in a variety of cancers but has yet to be explored at scale in osteosarcoma. Here we examined genome-wide DNA methylation patterns in 24 human and 44 canine osteosarcoma samples identifying groups of highly correlated DNA methylation marks in human and canine osteosarcoma samples. We also link specific DNA methylation patterns to key transcriptional programs in both human and canine osteosarcoma. Building on previous work, we built a DNA methylation-based measure for the presence and abundance of various immune cell types in osteosarcoma. Finally, we determined that the underlying state of the tumor, and not changes in cell composition, were the main driver of differences in DNA methylation across the human and canine samples.

SIGNIFICANCE:

Genome wide comparison of DNA methylation patterns in osteosarcoma across two species lays the ground work for the exploration of DNA methylation programs that help establish conserved transcriptional programs in the context of varied mutational landscapes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Ósseas / Osteossarcoma Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Ósseas / Osteossarcoma Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article