Antibody Aggregate Removal Using a Mixed-Mode Chromatography Resin.
Methods Mol Biol
; 2178: 345-354, 2021.
Article
em En
| MEDLINE
| ID: mdl-33128760
ABSTRACT
In monoclonal antibody (mAb) production, aggregates represent a major class of product-related impurities that needs to be removed by the downstream process. Protein A chromatography is generally less effective at removing antibody aggregates under typical conditions, and in most cases aggregate removal relies on a subsequent polishing chromatography. Here we describe a procedure for effective removal of antibody aggregates using the mixed-mode chromatography resin Capto MMC ImpRes. Clearance of aggregates was confirmed by analytical size-exclusion chromatography (SEC) and native gel electrophoresis.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteína Estafilocócica A
/
Agregados Proteicos
/
Anticorpos Monoclonais
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article