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Modification of late human embryo development after blastomere removal on day 3 for preimplantation genetic testing.
Lammers, Jenna; Reignier, Arnaud; Loubersac, Sophie; Chtourou, Sana; Lefebvre, Tiphaine; Barrière, Paul; Fréour, Thomas.
Afiliação
  • Lammers J; Service de Biologie et Médecine de la Reproduction, CHU Nantes, Nantes, France.
  • Reignier A; Centre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, Nantes, France.
  • Loubersac S; Service de Biologie et Médecine de la Reproduction, CHU Nantes, Nantes, France.
  • Chtourou S; Centre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, Nantes, France.
  • Lefebvre T; Faculté de Médecine, Université de Nantes, Nantes, France.
  • Barrière P; Service de Biologie et Médecine de la Reproduction, CHU Nantes, Nantes, France.
  • Fréour T; Centre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, Nantes, France.
Syst Biol Reprod Med ; 67(2): 121-126, 2021 Apr.
Article em En | MEDLINE | ID: mdl-33148055
The purpose of our study was to use a time-lapse monitoring (TLM) system to determine if day 3 blastomere biopsy for preimplantation genetic testing (PGT) had an impact on subsequent morphokinetic parameters at the morula and blastocyst stages. In this retrospective monocentric study conducted between May 2013 and August 2017, we compared late morphokinetic parameters in embryos undergoing day 3 blastomere biopsy for PGT and in control non-biopsied embryos obtained in intracytoplasmic sperm injection (ICSI) cycles for male infertility. All embryos in both groups were cultured in a TLM system. The biopsy group was composed of 1691 embryos (386 PGT cycles). The control group was composed of 2578 embryos (786 ICSI cycles). Early morphokinetic parameters up to day 3 were similar in both groups. Concerning late morphokinetic parameters, the onset of compaction (tSC), fully-compacted morula stage (tM), onset of cavitation/early blastulation (tSB), and blastocyst stages (tB and tEB) appeared significantly earlier in the biopsy group than in the control group. We found that late morphokinetic events at the morula and the blastocyst stages occurred significantly earlier in biopsied embryos than in control non-biopsied-embryos. The mechanisms underlying these modifications of embryo development after biopsy should be investigated in order to determine precisely, and this phenomenon could be associated with embryo, fetal, and offspring development.Abbreviations: TLM: time-lapse monitoring; PGT: preimplantation genetic testing; ICSI: intracytoplasmic sperm injection; tSC: the onset of compaction; tM: fully-compacted morula stage; tSB: onset of cavitation/early blastulation; tB and tEB: blastocyst stages; OHSS: ovarian hyperstimulation syndrome.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Blastômeros / Diagnóstico Pré-Implantação Tipo de estudo: Diagnostic_studies / Observational_studies Limite: Female / Humans / Male / Pregnancy Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Blastômeros / Diagnóstico Pré-Implantação Tipo de estudo: Diagnostic_studies / Observational_studies Limite: Female / Humans / Male / Pregnancy Idioma: En Ano de publicação: 2021 Tipo de documento: Article