Digital Imaging-based Colourimetry for Enzymatic Processes in Transparent Liquid Marbles.
Chemphyschem
; 22(1): 99-105, 2021 01 07.
Article
em En
| MEDLINE
| ID: mdl-33164308
ABSTRACT
Liquid marbles are a promising microreactor platform that recently attracts significant research interest owing to their ability to accommodate a wide range of micro reactions. However, the use of destructive and ex-situ methods to monitor reactions impairs the potential of liquid-marble-based microreactors. This paper proposes a non-destructive, inâ
situ, and cost-effective digital-imaging-based colourimetric monitoring method for transparent liquid marbles, using the enzymatic hydrolysis of starch as an illustrative example. The colourimetric reaction between starch and iodine produces a complex that exhibits a dark blue colour. We found that the absorbance of red channel of digital images showed a linear relationship with starch concentration with high sensitivity and repeatability. This digital-imaging-based colourimetric method was used to study the hydrolysis of starch by α-amylase. The results show high accuracy and applicability of first-order kinetics for this reaction. The demonstration of digital-imaging-based colourimetry indicates the potential of liquid marble-based microreactors.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carbonato de Cálcio
/
Colorimetria
/
Alfa-Amilases
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article