The gene encoding lycopene epsilon cyclase of celery enhanced lutein and ß-carotene contents and confers increased salt tolerance in Arabidopsis.
Plant Physiol Biochem
; 157: 339-347, 2020 Dec.
Article
em En
| MEDLINE
| ID: mdl-33186851
ABSTRACT
Celery (Apium graveolens L.) is a leafy vegetable of Apiaceae, which is greatly popular because of its rich nutrients. Lutein and ß-carotene are two important carotenoids. Lycopene epsilon cyclase (LCY-ε) is a key branch point enzyme in the carotenoid biosynthetic pathway. In this study, we cloned the AgLCY-ε gene from celery and overexpressed it in Arabidopsis. The results showed that both lutein and ß-carotene accumulation increased significantly in transgenic Arabidopsis hosting AgLCY-ε gene, compared with wild type (WT) plants. The transcription levels of AtPSY and AtCRTISO genes involved in carotenoids biosynthesis also increased in transgenic lines. One-month-old transgenic Arabidopsis seedlings were treated with 200 mM NaCl. The malondialdehyde (MDA) content in transgenic Arabidopsis plants after salt treatment was significantly lower, and the activities of the two antioxidant enzymes, superoxide dismutase (SOD) and peroxidase (POD), were significantly increased than that of WT plants. Overexpression of AgLCY-ε gene showed increased lutein and ß-carotene accumulations, and enhanced salt tolerance in transgenic plants.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Luteína
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Arabidopsis
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Beta Caroteno
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Liases Intramoleculares
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Tolerância ao Sal
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Apium
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article