Thiazole orange: a new dye for Plasmodium species analysis.
Cytometry
; 8(6): 568-70, 1987 Nov.
Article
em En
| MEDLINE
| ID: mdl-3322722
ABSTRACT
A rapid sensitive method for the determination of Plasmodium falciparum in in vitro culture is presented. The technique employs a fluorescent flow cytometer equipped with a 15-mwatt argon laser that emits light at 488 nm and a membrane-permeable fluorochrome thiazole orange (TO) that stains RNA. Parasitized red cells are stained by suspending them in 1 ml of phosphate-buffered saline (PBS) containing 10(-5) M of TO and incubating this mixture for 15 min in the dark at room temperature. The stained cells may be analyzed fresh or after fixation with 1% paraformaldehyde/PBS or 0.25% glutaraldehyde/PBS. Alternatively the cells may be fixed first and then stained. There is excellent correspondence between the number of fluorescent-labeled parasitized red cells and Giemsa-stained cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmodium falciparum
/
Tiazóis
/
Corantes Fluorescentes
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1987
Tipo de documento:
Article