Crystal structure of PYCH_01220 from Pyrococcus yayanosii potentially involved in binding nucleic acid.
Proteins
; 89(4): 468-472, 2021 04.
Article
em En
| MEDLINE
| ID: mdl-33236809
ABSTRACT
We report the crystal structure of PYCH_01220, a hypothetical protein in Pyrococcus yayanosii CH1. This protein is composed of two domains, named Domain A and Domain B. While Domain B is not significantly homologous to known protein structures, Domain A is structurally analogous to the C-terminal ribonuclease domain of Escherichia coli colicin D. Domain A has a positively charged surface patch rendered by 13 basic residues, eight arginine or lysine residues of which are evolutionarily conserved. Electrophoretic mobility shift assays showed that PYCH_01220 binds to DNA, and charge-inversion mutations on this patch negatively affect the DNA binding, suggesting that the function of PYCH_01220 might involve nucleic acid-binding via the positively charged patch.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Pyrococcus
/
Proteínas Arqueais
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article