The effect of transport temperature and time on the recovery of antimicrobial-resistant Enterobacterales in stool.
Diagn Microbiol Infect Dis
; 99(2): 115210, 2021 Feb.
Article
em En
| MEDLINE
| ID: mdl-33242837
ABSTRACT
Surveillance for antibiotic-resistant (AR) bacteria is challenging. We evaluated AR Enterobacterales survival in stool over various transport conditions. Stool in Cary-Blair medium was spiked with AR Enterobacterales, held at 3⯰C, 20⯰C, or 37⯰C, and cultured on days 3, 8, and 15. Stool from US international travelers sent through the US mail was also screened. We compared recovery rates using Fisher's exact tests and linear regression models. AR Enterobacterales recovery reduced with time (86% versus 75% versus 61% at days 3, 8, and 15; Beta for linear trend=-0.02, r2=0.99, P=0.02) and colder temperatures [56% (3⯰C) versus 89% (20⯰C) versus 86% (37⯰C); P=0.003]. Traveler sample recovery also reduced with transport time (Beta for linear trend=-0.03, r2=0.70, P=0.01) but not with season [20% (cold) versus 22% (warm), P=0.7]. AR Enterobacterales are found over variable transport conditions, providing rationale for expanding surveillance sample processing timelines.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Manejo de Espécimes
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Farmacorresistência Bacteriana
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Enterobacteriaceae
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Fezes
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article