Functional characterization of 67 endocytic accessory proteins using multiparametric quantitative analysis of CCP dynamics.

Bhave, Madhura; Mino, Rosa E; Wang, Xinxin; Lee, Jeon; Grossman, Heather M; Lakoduk, Ashley M; Danuser, Gaudenz; Schmid, Sandra L; Mettlen, Marcel

Bhave, Madhura; Mino, Rosa E; Wang, Xinxin; Lee, Jeon; Grossman, Heather M; Lakoduk, Ashley M; Danuser, Gaudenz; Schmid, Sandra L; Mettlen, Marcel.

Afiliação

Bhave M; Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390.
Mino RE; Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390.
Wang X; Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390.
Lee J; Lyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390.
Grossman HM; Lyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390.
Lakoduk AM; Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390.
Danuser G; Lyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390.
Schmid SL; Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390.
Mettlen M; Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390.

Proc Natl Acad Sci U S A ; 117(50): 31591-31602, 2020 12 15.

Article em En | MEDLINE | ID: mdl-33257546

Assuntos

Proteínas Adaptadoras de Transporte Vesicular/metabolismo; Membrana Celular/metabolismo; Clatrina/metabolismo; Invaginações Revestidas da Membrana Celular/metabolismo; Endocitose/fisiologia; Proteínas Adaptadoras de Transporte Vesicular/genética; Sistemas CRISPR-Cas/genética; Linhagem Celular; Análise por Conglomerados; Técnicas de Silenciamento de Genes; Proteínas de Fluorescência Verde/química; Proteínas de Fluorescência Verde/genética; Humanos; Microscopia Intravital/métodos; Substâncias Luminescentes/química; Microscopia de Fluorescência/métodos; Imagem Molecular/métodos; RNA Interferente Pequeno/metabolismo

Palavras-chave

CRISPRi screen; GAK; SNAREs; clathrin-mediated endocytosis; total internal reflection fluorescence microscopy

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Membrana Celular / Clatrina / Invaginações Revestidas da Membrana Celular / Proteínas Adaptadoras de Transporte Vesicular / Endocitose Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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