Transcription factor STAT1 promotes the proliferation, migration and invasion of nasopharyngeal carcinoma cells by upregulating LINC01160.
Future Oncol
; 17(1): 57-69, 2021 Jan.
Article
em En
| MEDLINE
| ID: mdl-33263259
ABSTRACT
Aim:
To investigate the role of LINC01160 in nasopharyngeal carcinoma (NPC). Materials &methods:
Using NPC cells CNE-2 and HNE-2 in vitro, we performed quantitative PCR to determine mRNA expression and western blotting to determine protein expression. CCK-8, transwell, flow cytometry and wound healing assays were done to examine the function of LINC01160 and STAT1. Chromatin immunoprecipitation PCR (ChIP-PCR) confirmed that STAT1 combines with the LINC01160 promoter region. Xenograft experiments were used to verify the role of STAT1 and LINC01160 in vivo.Results:
LINC01160 is upregulated in NPC and can promote a malignant cell phenotype. STAT1 is a transcription factor of LINC01160 and can promote a malignant cell phenotype through upregulating LINC01160 expression.Conclusion:
STAT1 can promote a malignant cell phenotype by upregulating LINC01160.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regulação Neoplásica da Expressão Gênica
/
Neoplasias Nasofaríngeas
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Fator de Transcrição STAT1
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RNA Longo não Codificante
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Carcinoma Nasofaríngeo
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Female
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Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article