Effects of TGF-ß1 on the expression of endometrial stromal cell-related protein and mRNA.
Eur Rev Med Pharmacol Sci
; 24(22): 11475-11480, 2020 11.
Article
em En
| MEDLINE
| ID: mdl-33275213
ABSTRACT
OBJECTIVE:
To investigate the effects of transforming growth factor ß1 (TGF-ß1) on α-smooth muscle actin (α-SMA), insulin-like growth factor I (IGF-I), and type I collagen (Col I) expression in endometrial stromal cells as well as on fibronectin (FN) level. PATIENTS ANDMETHODS:
56 patients with normal endometrial tissue obtained from surgery were selected from June 2018 to November 2019. Endometrial stromal cells were isolated from patients and then assigned to the control group and observation group (addition of TGF-ß1) followed by the analysis of cellular activity by Thiazole blue staining; and α-SMA, IGF-I, Col I, and FN mRNA and protein levels by real-time fluorescent PCR and Western blot.RESULTS:
The cell proliferation rate at 12 h, 24 h, 36 h, and 72 h after culture in both groups was higher than 0 h (p < 0.05) with higher cell proliferation in the observation group than the control group (p < 0.05). Real-time fluorescence PCR results showed that the levels of α-SMA, IGF-I, Col I, and FN mRNA in endometrial stromal cells of the observation group after TGF-ß1 intervention were higher than those in the control group (p < 0.05). Meanwhile, α-SMA, IGF-I, Col I, and FN protein level was also elevated in the observation group after TGF-ß1 treatment (p < 0.05).CONCLUSIONS:
TGF-ß1 can stimulate the proliferation of endometrial stromal cells, which may be related to regulate α-SMA, IGF-I, Col I, and FN expression.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
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Fator de Crescimento Insulin-Like I
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Actinas
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Fibronectinas
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Colágeno Tipo I
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Fator de Crescimento Transformador beta1
Limite:
Adult
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Female
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Humans
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Middle aged
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article