Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis.

Louradour, Isabelle; Ghosh, Kashinath; Inbar, Ehud; Sacks, David L; Aluvihare, Channa; Harrell, Robert A

Louradour, Isabelle; Ghosh, Kashinath; Inbar, Ehud; Sacks, David L; Aluvihare, Channa; Harrell, Robert A.

Afiliação

Louradour I; Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health.
Ghosh K; Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health.
Inbar E; Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health.
Sacks DL; Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health.
Aluvihare C; University of Maryland Insect Transformation Facility, The Institute for Bioscience and Biotechnology Research.
Harrell RA; University of Maryland Insect Transformation Facility, The Institute for Bioscience and Biotechnology Research; harrelr@umd.edu.

J Vis Exp ; (165)2020 11 17.

Article em En | MEDLINE | ID: mdl-33283789

Assuntos

Proteína 9 Associada à CRISPR/metabolismo; Sistemas CRISPR-Cas/genética; Embrião não Mamífero/metabolismo; Microinjeções; Mutagênese/genética; Phlebotomus/embriologia; Animais; Feminino; Masculino; Camundongos; Microtecnologia; Mutação/genética; Agulhas; Phlebotomus/genética; Phlebotomus/imunologia; Phlebotomus/parasitologia

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Phlebotomus / Mutagênese / Embrião não Mamífero / Sistemas CRISPR-Cas / Proteína 9 Associada à CRISPR / Microinjeções Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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