Chromatin Immunoprecipitation (ChIP) from Mouse Liver Nuclei.
Methods Mol Biol
; 2130: 115-125, 2021.
Article
em En
| MEDLINE
| ID: mdl-33284440
ABSTRACT
Understanding the binding of regulatory proteins to their cognate genomic sites is an important step in deciphering transcriptional networks such as the circadian oscillator. Chromatin immunoprecipitation (ChIP) enables the detection and temporal analysis of such binding events in vivo. Here, we describe the individual steps from the generation of formaldehyde-cross-linked chromatin from mouse liver nuclei, fragmentation thereof, immunoprecipitation, reversal of cross-links, fragment cleanup, and detection of binding sites by real-time PCR. Depending on the quality of the employed antibody, a clear enrichment signal over the background is expected with a resolution of about 500-800 base pairs around the selected primer-probe pair.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatina
/
Imunoprecipitação da Cromatina
/
Fígado
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article