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Antigenicity of hypothetical protein HP33 of Vibrio harveyi Y6 causing scale drop and muscle necrosis disease in Asian sea bass.
Kwankijudomkul, Anusara; Dong, Ha Thanh; Longyant, Siwaporn; Sithigorngul, Paisarn; Khunrae, Pongsak; Rattanarojpong, Triwit; Senapin, Saengchan.
Afiliação
  • Kwankijudomkul A; Department of Microbiology, Faculty of Science, King Mongkut's University of Technology, Thonburi (KMUTT), Bangkok, 10140, Thailand.
  • Dong HT; Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok, 10300, Thailand.
  • Longyant S; Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand.
  • Sithigorngul P; Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand.
  • Khunrae P; Department of Microbiology, Faculty of Science, King Mongkut's University of Technology, Thonburi (KMUTT), Bangkok, 10140, Thailand.
  • Rattanarojpong T; Department of Microbiology, Faculty of Science, King Mongkut's University of Technology, Thonburi (KMUTT), Bangkok, 10140, Thailand. Electronic address: triwit.rat@kmutt.ac.th.
  • Senapin S; Fish Health Platform, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Bangkok, 10400, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA),
Fish Shellfish Immunol ; 108: 73-79, 2021 Jan.
Article em En | MEDLINE | ID: mdl-33285163
A unique strain of Vibrio harveyi is the causative agent of scale drop and muscle necrosis disease (SDMND) in Asian sea bass (Lates calcarifer). This study investigated the protein profiles of SDMND-causing Vibrio harveyi isolates compared to the reference V. harveyi ATCC 14126 strain. A distinct protein band of 33 kDa, namely HP33, found from only V. harveyi SDMND was subjected to analysis by LC-MS/MS and the identified peptide sequences matched to an unknown hypothetical protein. Detection of HP33 coding sequence was investigated at both genomic and transcriptional levels and the results consistently supported the protein analysis. Recombinant HP33 protein was then produced using Escherichia coli system. The rHP33 protein did not cause mortality or visible clinical signs to Asian sea bass. However, the rHP33 protein was able to stimulate antibody response in Asian sea bass as evidenced by Western blotting and agglutination tests. Here, we proposed that rHP33 might be a good protein target for development of subunit vaccine and/or immunostimulant to protect Asian sea bass from SDMND.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Vibrio / Vibrioses / Bass / Doenças dos Peixes / Imunogenicidade da Vacina / Necrose Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Vibrio / Vibrioses / Bass / Doenças dos Peixes / Imunogenicidade da Vacina / Necrose Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article