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TCRα reporter mice reveal contribution of dual TCRα expression to T cell repertoire and function.
Yang, Letitia; Jama, Burhan; Wang, Huawei; Labarta-Bajo, Lara; Zúñiga, Elina I; Morris, Gerald P.
Afiliação
  • Yang L; Department of Pathology, University of California San Diego, La Jolla, CA 92093.
  • Jama B; Department of Pathology, University of California San Diego, La Jolla, CA 92093.
  • Wang H; Department of Pathology, University of California San Diego, La Jolla, CA 92093.
  • Labarta-Bajo L; Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093.
  • Zúñiga EI; Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093.
  • Morris GP; Department of Pathology, University of California San Diego, La Jolla, CA 92093; gpmorris@ucsd.edu.
Proc Natl Acad Sci U S A ; 117(51): 32574-32583, 2020 12 22.
Article em En | MEDLINE | ID: mdl-33288689
It is known that a subpopulation of T cells expresses two T cell receptor (TCR) clonotypes, though the extent and functional significance of this is not established. To definitively evaluate dual TCRα cells, we generated mice with green fluorescent protein and red fluorescent protein reporters linked to TCRα, revealing that ∼16% of T cells express dual TCRs, notably higher than prior estimates. Importantly, dual TCR expression has functional consequences, as dual TCR cells predominated response to lymphocytic choriomeningitis virus infection, comprising up to 60% of virus-specific CD4+ and CD8+ T cells during acute responses. Dual receptor expression selectively influenced immune memory, as postinfection memory CD4+ populations contained significantly increased frequencies of dual TCR cells. These data reveal a previously unappreciated contribution of dual TCR cells to the immune repertoire and highlight their potential effects on immune responses.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos T / Genes Codificadores da Cadeia alfa de Receptores de Linfócitos T / Coriomeningite Linfocítica Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos T / Genes Codificadores da Cadeia alfa de Receptores de Linfócitos T / Coriomeningite Linfocítica Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article