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Circulating MicroRNA Profiling Reveals Specific Subsignatures in Response to a Maximal Incremental Exercise Test.
Fernández-Sanjurjo, Manuel; Díaz-Martínez, Ángel E; Díez-Robles, Sergio; González-González, Felipe; de Gonzalo-Calvo, David; Rabadán, Manuel; Dávalos, Alberto; Fernández-García, Benjamín; Iglesias-Gutiérrez, Eduardo.
Afiliação
  • Fernández-Sanjurjo M; Department of Functional Biology, University of Oviedo, Oviedo, Spain.
  • Díaz-Martínez ÁE; Area of Metabolism, Health Research Institute of the Principality of Asturias (ISPA), Oviedo, Spain.
  • Díez-Robles S; Clinical Laboratory, Sports Medicine Center, Spanish Sports Health Protection Agency, Spanish Government, Madrid, Spain.
  • González-González F; Department of Functional Biology, University of Oviedo, Oviedo, Spain.
  • de Gonzalo-Calvo D; Department of Functional Biology, University of Oviedo, Oviedo, Spain.
  • Rabadán M; Translational Research in Respiratory Medicine, Arnau de Vilanova-Santa University Hospital Maria, IRBLleida, Lleida, Spain.
  • Dávalos A; CIBER of Respiratory Diseases (CIBERES), Institute of Health Carlos III, Madrid, Spain.
  • Fernández-García B; Physiology of Effort Service, Sports Medicine Center, Spanish Sports Health Protection Agency, Spanish Government, Madrid, Spain.
  • Iglesias-Gutiérrez E; Laboratory of Epigenetics of Lipid Metabolism, IMDEA Food Institute, CEI UAM + CSIC, Madrid, Spain; and.
J Strength Cond Res ; 35(2): 287-291, 2021 Feb 01.
Article em En | MEDLINE | ID: mdl-33337695
ABSTRACT
ABSTRACT Fernández-Sanjurjo, M, Díaz-Martínez, ÁE, Díez-Robles, S, González-González, F, de Gonzalo-Calvo, D, Rabadán, M, Dávalos, A, Fernández-García, B, and Iglesias-Gutiérrez, E. Circulating microRNA profiling reveals specific subsignatures in response to a maximal incremental exercise test. J Strength Cond Res 35(2) 287-291, 2021-Circulating microRNAs (c-miRNAs) have been described as emergent regulators and biomarkers of exercise. The aim of this study was to analyze the c-miRNA response to a maximal incremental exercise test (MIET) and its relationship with markers of exercise response and adaptation. Two blood samples were collected from 9 male amateur runners (31-50 years), before (Pre) and after (Post) a MIET. The maximal oxygen uptake (V̇o2max), maximum heart rate (HRmax), and maximal aerobic speed (MAS) were recorded. Lactate and creatine kinase (CK) plasma concentrations were measured. A panel of 752 miRNAs was analyzed using standardized protocols and relative quantification to Pre. A total of 13 miRNAs were found significantly upregulated at Post. By focusing on the exercise markers that correlate with the expression of these miRNAs, they were clustered into different functional groups or subsignatures. Thus, miR-21-5p, miR-29b-3p, and miR-183-5p showed a strong correlation with HRmax and a validated target signature related to fatty acid metabolism. Furthermore, let-7c-5p, miR-340-5p, miR-425-3p, and miR-629-5p were significantly correlated with CK, and the most significantly enriched pathways for these subsignatures were the Hippo signaling pathway and signaling pathways regulating pluripotency of stem cells. Finally, Pre miR-106b-5p expression showed an inverse association with MAS and Post lactate concentration, which highlights its relevance as biomarker of training status and its predictive value for performance. No significant correlations were observed with V̇o2max. Our results define for the first time specific functional c-miRNA subsignatures, adding novel evidence about their potential regulatory role in exercise response.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: MicroRNAs / MicroRNA Circulante Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: MicroRNAs / MicroRNA Circulante Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article