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Characterization and phylogenetic evolution of mitochondrial genome in Tibetan chicken.
Jia, Xiaoxu; Lu, Junxian; Tang, Xiujun; Fan, Yanfeng; Ge, Qinglian; Gao, Yushi.
Afiliação
  • Jia X; Jiangsu Institute of Poultry Science, Yangzhou, PR China.
  • Lu J; Jiangsu Institute of Poultry Science, Yangzhou, PR China.
  • Tang X; Jiangsu Institute of Poultry Science, Yangzhou, PR China.
  • Fan Y; Nanjing Agricultural University, Nanjing, PR China.
  • Ge Q; Jiangsu Institute of Poultry Science, Yangzhou, PR China.
  • Gao Y; Jiangsu Institute of Poultry Science, Yangzhou, PR China.
Anim Biotechnol ; 33(6): 1371-1377, 2022 Nov.
Article em En | MEDLINE | ID: mdl-33347342
ABSTRACT
The objective of this study was to characterize mitochondrial genome and investigate phylogenetic evolution in Tibetan chicken. In this study, four haplotypes were identified based on D-loop sequencing in Tibetan chicken (n = 40), and each representative of four haplotypes was selected for total mitochondrial genome sequencing and analyzed together with published mitochondrial genome data of red jungle fowl. Four haplotypes belonged to three previously published clades, i.e., Clade A, clade B and clade E. Based on D-loop sequencing data, the average haplotype diversity and nucleotide diversity were 0.658 ± 0.065 and 0.00442 ± 0.00094, respectively. The mitochondrial genome of Tibetan chicken is 16,785 bp in size, consisting of 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, 13 protein-coding genes and one non-coding control region (CR). Compared with the mitochondrial genome, a phylogenetic tree based on the D-loop sequence had a messy distribution, and no breed cluster pattern was observed in Tibetan chicken. The results indicate that Tibetan chicken populations in our study have relatively low nucleotide and haplotype diversity and likely share multiple maternal lineages. The D-loop sequence has limited power for the resolution of phylogenetic relationships in comparison with the complete mitochondrial genome.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Genoma Mitocondrial Limite: Animals País/Região como assunto: Asia Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Genoma Mitocondrial Limite: Animals País/Região como assunto: Asia Idioma: En Ano de publicação: 2022 Tipo de documento: Article