Short communication: Genotype-phenotype association analysis revealed different utilization ability of 2'-fucosyllactose in Bifidobacterium genus.
J Dairy Sci
; 104(2): 1518-1523, 2021 Feb.
Article
em En
| MEDLINE
| ID: mdl-33358168
ABSTRACT
The oligosaccharide 2'-fucosyllactose (2'FL) in human breast milk selectively promotes the proliferation of bifidobacteria. One hundred fifty-one Bifidobacterium strains were evaluated for their capacity to utilize 2'FL based on the combination of phenotype and genotype association analysis. Through genotype analysis, 37 strains were predicted to have the ability to use 2'FL, including Bifidobacteriumbifidum, Bifidobacteriumbreve, Bifidobacteriumlongum ssp. longum, Bifidobacteriumlongum ssp. infantis, and Bifidobacteriumdentium, whereas Bifidobacteriumadolescentis, Bifidobacteriumanimalis, Bifidobacteriumpseudocatenulatum, and Bifidobacteriumangulatum could not use 2'FL. For in vitro utilization, there were noteworthy differences for 2'FL usage among different species, which were 100% consistent with genotype prediction. The results indicated that 2'FL utilization ability differed even within the same species, and Bifidobacterium followed the currently well-known pathway to utilize 2'FL, which could provide guidance to develop personalized prebiotics for different bifidobacteria via gene-trait matching analysis.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trissacarídeos
/
Bifidobacterium
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Leite Humano
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article