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Insulin resistance is mechanistically linked to hepatic mitochondrial remodeling in non-alcoholic fatty liver disease.
Shannon, Chris E; Ragavan, Mukundan; Palavicini, Juan Pablo; Fourcaudot, Marcel; Bakewell, Terry M; Valdez, Ivan A; Ayala, Iriscilla; Jin, Eunsook S; Madesh, Muniswamy; Han, Xianlin; Merritt, Matthew E; Norton, Luke.
Afiliação
  • Shannon CE; Division of Diabetes, University of Texas Health Science Center and Texas Diabetes Institute, San Antonio, TX, USA.
  • Ragavan M; Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, FL, USA.
  • Palavicini JP; Division of Diabetes, University of Texas Health Science Center and Texas Diabetes Institute, San Antonio, TX, USA; Barshop Institute for Longevity and Aging Studies, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
  • Fourcaudot M; Division of Diabetes, University of Texas Health Science Center and Texas Diabetes Institute, San Antonio, TX, USA.
  • Bakewell TM; Division of Diabetes, University of Texas Health Science Center and Texas Diabetes Institute, San Antonio, TX, USA.
  • Valdez IA; Division of Diabetes, University of Texas Health Science Center and Texas Diabetes Institute, San Antonio, TX, USA.
  • Ayala I; Division of Diabetes, University of Texas Health Science Center and Texas Diabetes Institute, San Antonio, TX, USA.
  • Jin ES; Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX, USA.
  • Madesh M; Division of Nephrology, University of Texas Health Science Center and Texas Diabetes Institute, San Antonio, TX, USA.
  • Han X; Division of Diabetes, University of Texas Health Science Center and Texas Diabetes Institute, San Antonio, TX, USA; Barshop Institute for Longevity and Aging Studies, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA; Glenn Biggs Institute for Alzheimer's & Neurodege
  • Merritt ME; Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, FL, USA.
  • Norton L; Division of Diabetes, University of Texas Health Science Center and Texas Diabetes Institute, San Antonio, TX, USA. Electronic address: nortonl@uthscsa.edu.
Mol Metab ; 45: 101154, 2021 03.
Article em En | MEDLINE | ID: mdl-33359401
OBJECTIVE: Insulin resistance and altered hepatic mitochondrial function are central features of type 2 diabetes (T2D) and non-alcoholic fatty liver disease (NAFLD), but the etiological role of these processes in disease progression remains unclear. Here we investigated the molecular links between insulin resistance, mitochondrial remodeling, and hepatic lipid accumulation. METHODS: Hepatic insulin sensitivity, endogenous glucose production, and mitochondrial metabolic fluxes were determined in wild-type, obese (ob/ob) and pioglitazone-treatment obese mice using a combination of radiolabeled tracer and stable isotope NMR approaches. Mechanistic studies of pioglitazone action were performed in isolated primary hepatocytes, whilst molecular hepatic lipid species were profiled using shotgun lipidomics. RESULTS: Livers from obese, insulin-resistant mice displayed augmented mitochondrial content and increased tricarboxylic acid cycle (TCA) cycle and pyruvate dehydrogenase (PDH) activities. Insulin sensitization with pioglitazone mitigated pyruvate-driven TCA cycle activity and PDH activation via both allosteric (intracellular pyruvate availability) and covalent (PDK4 and PDP2) mechanisms that were dependent on PPARγ activity in isolated primary hepatocytes. Improved mitochondrial function following pioglitazone treatment was entirely dissociated from changes in hepatic triglycerides, diacylglycerides, or fatty acids. Instead, we highlight a role for the mitochondrial phospholipid cardiolipin, which underwent pathological remodeling in livers from obese mice that was reversed by insulin sensitization. CONCLUSION: Our findings identify targetable mitochondrial features of T2D and NAFLD and highlight the benefit of insulin sensitization in managing the clinical burden of obesity-associated disease.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Resistência à Insulina / Mitocôndrias Hepáticas / Hepatopatia Gordurosa não Alcoólica / Fígado / Mitocôndrias Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Resistência à Insulina / Mitocôndrias Hepáticas / Hepatopatia Gordurosa não Alcoólica / Fígado / Mitocôndrias Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article