S-glutathionylation of the Na+-K+ Pump: A Novel Redox Mechanism in Preeclampsia.
J Clin Endocrinol Metab
; 106(4): 1091-1100, 2021 03 25.
Article
em En
| MEDLINE
| ID: mdl-33382878
ABSTRACT
CONTEXT Reduced Na+-K+ pump activity is widely reported in preeclampsia and may be caused by a reversible oxidative modification that is a novel pathological feature of preeclampsia. OBJECTIVE:
This work aims to determine whether ßâ1 subunit (GSS-ßâ1) protein glutathionylation of the Na+-K + pump occurs in preeclampsia.METHODS:
The GSS-ß1 of the Na+-K+ pump and its subunit expression in human placentas were compared between women with healthy pregnancies and women with preeclampsia. Human placental samples of pregnant women with preeclampsia (n = 11, mean gestational age 36.5 weeks) were used to examine the GSS-ßâ1 of the Na+-K+ pump, compared to healthy pregnancies (n = 11, mean gestational age 39 weeks).The potential pathogenetic role of GSS-ßâ1-mediated Na+-K+ pump dysfunction in preeclampsia was investigated.RESULTS:
Protein expression of the ßâ1 subunit was unchanged in placentas from women with preeclampsia vs those with normotensive pregnancies. Preeclamptic placentas had a significantly increased GSS-ßâ1 of the Na+-K+ pump compared to those from healthy pregnancies, and this was linked to a decrease in αâ1/ßâ1 subunit coimmunoprecipitation. The cytosolic p47phox nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase subunit and its coimmunoprecipitation with the αâ1 Na+-K+ pump subunit was increased in preeclamptic placentas, thus implicating NADPH oxidase-dependent pump inhibition.CONCLUSIONS:
The high level of ßâ1 pump subunit glutathionylation provides new insights into the mechanism of Na+-K+ pump dysfunction in preeclampsia.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pré-Eclâmpsia
/
ATPase Trocadora de Sódio-Potássio
/
Glutationa
Tipo de estudo:
Observational_studies
Limite:
Adult
/
Female
/
Humans
/
Pregnancy
País/Região como assunto:
Oceania
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article