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DNA Nanoswitch Barcodes for Multiplexed Biomarker Profiling.
Chandrasekaran, Arun Richard; MacIsaac, Molly; Vilcapoma, Javier; Hansen, Clinton H; Yang, Darren; Wong, Wesley P; Halvorsen, Ken.
Afiliação
  • Chandrasekaran AR; The RNA Institute, University at Albany, State University of New York, New York, New York 12222, United States.
  • MacIsaac M; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, Massachusetts 02115, United States.
  • Vilcapoma J; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, United States.
  • Hansen CH; Department of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, Massachusetts 02115, United States.
  • Yang D; The RNA Institute, University at Albany, State University of New York, New York, New York 12222, United States.
  • Wong WP; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, Massachusetts 02115, United States.
  • Halvorsen K; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, United States.
Nano Lett ; 21(1): 469-475, 2021 01 13.
Article em En | MEDLINE | ID: mdl-33395311
Molecular biomarkers play a key role in the clinic, aiding in diagnostics and prognostics, and in the research laboratory, contributing to our basic understanding of diseases. Detecting multiple and diverse molecular biomarkers within a single accessible assay would have great utility, providing a more comprehensive picture for clinical evaluation and research, but is a challenge with standard methods. Here, we report programmable DNA nanoswitches for multiplexed detection of up to 6 biomarkers at once with each combination of biomarkers producing a unique barcode signature among 64 possibilities. As a defining feature of our method, we show "mixed multiplexing" for simultaneous barcoded detection of different types of biomolecules, for example, DNA, RNA, antibody, and protein in a single assay. To demonstrate clinical potential, we show multiplexed detection of a prostate cancer biomarker panel in serum that includes two microRNA sequences and prostate specific antigen.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / MicroRNAs Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / MicroRNAs Idioma: En Ano de publicação: 2021 Tipo de documento: Article