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Osteogenic Properties of 3D-Printed Silica-Carbon-Calcite Composite Scaffolds: Novel Approach for Personalized Bone Tissue Regeneration.
Memarian, Parastoo; Sartor, Francesco; Bernardo, Enrico; Elsayed, Hamada; Ercan, Batur; Delogu, Lucia Gemma; Zavan, Barbara; Isola, Maurizio.
Afiliação
  • Memarian P; Department of Animal Medicine, Productions and Health, University of Padova, 35020 Legnaro, Italy.
  • Sartor F; Department of Animal Medicine, Productions and Health, University of Padova, 35020 Legnaro, Italy.
  • Bernardo E; Department of Industrial Engineering, University of Padova, 35131 Padova, Italy.
  • Elsayed H; Department of Industrial Engineering, University of Padova, 35131 Padova, Italy.
  • Ercan B; Refractories, Ceramics and Building Materials Department, National Research Centre, El-Bohous Str., 12622 Cairo, Egypt.
  • Delogu LG; Department of Metallurgical and Materials Engineering, Middle East Technical University, 06800 Ankara, Turkey.
  • Zavan B; Biomedical Engineering Program, Middle East Technical University, 06800 Ankara, Turkey.
  • Isola M; BIOMATEN, METU Center of Excellence in Biomaterials and Tissue Engineering, 06800 Ankara, Turkey.
Int J Mol Sci ; 22(2)2021 Jan 06.
Article em En | MEDLINE | ID: mdl-33418865
Carbon enriched bioceramic (C-Bio) scaffolds have recently shown exceptional results in terms of their biological and mechanical properties. The present study aims at assessing the ability of the C-Bio scaffolds to affect the commitment of canine adipose-derived mesenchymal stem cells (cAD-MSCs) and investigating the influence of carbon on cell proliferation and osteogenic differentiation of cAD-MSCs in vitro. The commitment of cAD-MSCs to an osteoblastic phenotype has been evaluated by expression of several osteogenic markers using real-time PCR. Biocompatibility analyses through 3-(4,5-dimethyl- thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), lactate dehydrogenase (LDH) activity, hemolysis assay, and Ames test demonstrated excellent biocompatibility of both materials. A significant increase in the extracellular alkaline phosphatase (ALP) activity and expression of runt-related transcription factor (RUNX), ALP, osterix (OSX), and receptor activator of nuclear factor kappa-Β ligand (RANKL) genes was observed in C-Bio scaffolds compared to those without carbon (Bio). Scanning electron microscopy (SEM) demonstrated excellent cell attachment on both material surfaces; however, the cellular layer on C-Bio fibers exhibited an apparent secretome activity. Based on our findings, graphene can improve cell adhesion, growth, and osteogenic differentiation of cAD-MSCs in vitro. This study proposed carbon as an additive for a novel three-dimensional (3D)-printable biocompatible scaffold which could become the key structural material for bone tissue reconstruction.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osso e Ossos / Regeneração Óssea / Engenharia Tecidual / Alicerces Teciduais Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osso e Ossos / Regeneração Óssea / Engenharia Tecidual / Alicerces Teciduais Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article