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High Integrity and Fidelity of Long-Term Cryopreserved Umbilical Cord Blood for Transplantation.
Kim, Gee-Hye; Kwak, Jihye; Kim, Sung Hee; Kim, Hee Jung; Hong, Hye Kyung; Jin, Hye Jin; Choi, Soo Jin; Oh, Wonil; Um, Soyoun.
Afiliação
  • Kim GH; Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do 13494, Korea.
  • Kwak J; Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do 13494, Korea.
  • Kim SH; Cord Blood Bank, MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do 13494, Korea.
  • Kim HJ; Cord Blood Bank, MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do 13494, Korea.
  • Hong HK; Cord Blood Bank, MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do 13494, Korea.
  • Jin HJ; Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do 13494, Korea.
  • Choi SJ; Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do 13494, Korea.
  • Oh W; Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do 13494, Korea.
  • Um S; Cord Blood Bank, MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do 13494, Korea.
J Clin Med ; 10(2)2021 Jan 14.
Article em En | MEDLINE | ID: mdl-33466868
ABSTRACT
Umbilical cord blood (UCB) is used as a source of donor cells for hematopoietic stem cell (HSC) transplantation. The success of transplantation is dependent on the quality of cord blood (CB) units for maximizing the chance of engraftment. Improved outcomes following transplantation are associated with certain factors of cryopreserved CB units total volume and total nucleated cell (TNC) count, mononuclear cell (MNC) count, and CD34+ cell count. The role of the storage period of CB units in determining the viability and counts of cells is less clear and is related to the quality of cryopreserved CB units. Herein, we demonstrate the recovery of viable TNCs and CD34+ cells, as well as the MNC viability in 20-year-old cryopreserved CB units in a CB bank (MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do, Korea). In addition, cell populations in CB units were evaluated for future clinical applications. The stable recovery rate of the viability of cryopreserved CB that had been stored for up to 20 years suggested the possibility of uses of the long-term cryopreservation of CB units. Similar relationships were observed in the recovery of TNCs and CD34+ cells in units of cryopreserved and fresh CB. The high-viability recovery of long-term cryopreserved CB suggests that successful hematopoietic stem cell (HSC) transplantation and other clinical applications, which are suitable for treating incurable diseases, may be performed regardless of long-term storage.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article