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3D Cell-Printed Hypoxic Cancer-on-a-Chip for Recapitulating Pathologic Progression of Solid Cancer.
Park, Wonbin; Bae, Mihyeon; Hwang, Minseon; Jang, Jinah; Cho, Dong-Woo; Yi, Hee-Gyeong.
Afiliação
  • Park W; Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH).
  • Bae M; Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH).
  • Hwang M; Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH).
  • Jang J; Department of Creative IT Engineering, Pohang University of Science and Technology (POSTECH); jinahjang@postech.ac.kr.
  • Cho DW; Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH); dwcho@postech.ac.kr.
  • Yi HG; Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH); Department of Rural and Biosystems Engineering, College of Agriculture and Life Sciences, Chonnam National University; hgyi@jnu.ac.kr.
J Vis Exp ; (167)2021 01 05.
Article em En | MEDLINE | ID: mdl-33491677
Cancer microenvironment has a significant impact on the progression of the disease. In particular, hypoxia is the key driver of cancer survival, invasion, and chemoresistance. Although several in vitro models have been developed to study hypoxia-related cancer pathology, the complex interplay of the cancer microenvironment observed in vivo has not been reproduced yet owing to the lack of precise spatial control. Instead, 3D biofabrication approaches have been proposed to create microphysiological systems for better emulation of cancer ecology and accurate anticancer treatment evaluation. Herein, we propose a 3D cell-printing approach to fabricate a hypoxic cancer-on-a-chip. The hypoxia-inducing components in the chip were determined based on a computer simulation of the oxygen distribution. Cancer-stroma concentric rings were printed using bioinks containing glioblastoma cells and endothelial cells to recapitulate a type of solid cancer. The resulting chip realized central hypoxia and aggravated malignancy in cancer with the formation of representative pathophysiological markers. Overall, the proposed approach for creating a solid-cancer-mimetic microphysiological system is expected to bridge the gap between in vivo and in vitro models for cancer research.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Progressão da Doença / Dispositivos Lab-On-A-Chip / Impressão Tridimensional / Hipóxia Tumoral / Neoplasias Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Progressão da Doença / Dispositivos Lab-On-A-Chip / Impressão Tridimensional / Hipóxia Tumoral / Neoplasias Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article