CRISPR/dCas9-mediated transposition with specificity and efficiency of site-directed genomic insertions.

Goshayeshi, Lena; Yousefi Taemeh, Sara; Dehdilani, Nima; Nasiri, Mohammadreza; Ghahramani Seno, Mohammad M; Dehghani, Hesam

Goshayeshi, Lena; Yousefi Taemeh, Sara; Dehdilani, Nima; Nasiri, Mohammadreza; Ghahramani Seno, Mohammad M; Dehghani, Hesam.

Afiliação

Goshayeshi L; Division of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Yousefi Taemeh S; Stem Cell Biology and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
Dehdilani N; Division of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Nasiri M; Stem Cell Biology and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
Ghahramani Seno MM; Division of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Dehghani H; Stem Cell Biology and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.

FASEB J ; 35(2): e21359, 2021 02.

Article em En | MEDLINE | ID: mdl-33496003

Assuntos

Sistemas CRISPR-Cas; Técnicas de Introdução de Genes/métodos; Proteína 9 Associada à CRISPR/metabolismo; Genes Reporter; Células HEK293; Humanos; Mutagênese Insercional; RNA Guia de Cinetoplastídeos/metabolismo; Transposases/genética; Transposases/metabolismo

Palavras-chave

CRISPR-associated protein 9; Insertional mutagenesis; gene insertion; piggyBac transposase; transposases

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas de Introdução de Genes / Sistemas CRISPR-Cas Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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