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Two mechanisms drive pronuclear migration in mouse zygotes.
Scheffler, Kathleen; Uraji, Julia; Jentoft, Ida; Cavazza, Tommaso; Mönnich, Eike; Mogessie, Binyam; Schuh, Melina.
Afiliação
  • Scheffler K; Department of Meiosis, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
  • Uraji J; School of Biochemistry, University of Bristol, Bristol, UK.
  • Jentoft I; Department of Meiosis, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
  • Cavazza T; Department of Meiosis, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
  • Mönnich E; Department of Meiosis, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
  • Mogessie B; Department of Meiosis, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
  • Schuh M; School of Biochemistry, University of Bristol, Bristol, UK.
Nat Commun ; 12(1): 841, 2021 02 05.
Article em En | MEDLINE | ID: mdl-33547291
A new life begins with the unification of the maternal and paternal chromosomes upon fertilization. The parental chromosomes first become enclosed in two separate pronuclei near the surface of the fertilized egg. The mechanisms that then move the pronuclei inwards for their unification are only poorly understood in mammals. Here, we report two mechanisms that act in concert to unite the parental genomes in fertilized mouse eggs. The male pronucleus assembles within the fertilization cone and is rapidly moved inwards by the flattening cone. Rab11a recruits the actin nucleation factors Spire and Formin-2 into the fertilization cone, where they locally nucleate actin and further accelerate the pronucleus inwards. In parallel, a dynamic network of microtubules assembles that slowly moves the male and female pronuclei towards the cell centre in a dynein-dependent manner. Both mechanisms are partially redundant and act in concert to unite the parental pronuclei in the zygote's centre.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Zigoto / Núcleo Celular / Proteínas rab de Ligação ao GTP / Fertilização / Forminas / Proteínas dos Microfilamentos / Proteínas do Tecido Nervoso Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Zigoto / Núcleo Celular / Proteínas rab de Ligação ao GTP / Fertilização / Forminas / Proteínas dos Microfilamentos / Proteínas do Tecido Nervoso Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article