Generation of cell-permeant recombinant human transcription factor GATA4 from E. coli.

Haridhasapavalan, Krishna Kumar; Sundaravadivelu, Pradeep Kumar; Bhattacharyya, Srirupa; Ranjan, Sujal Harsh; Raina, Khyati; Thummer, Rajkumar P

Haridhasapavalan, Krishna Kumar; Sundaravadivelu, Pradeep Kumar; Bhattacharyya, Srirupa; Ranjan, Sujal Harsh; Raina, Khyati; Thummer, Rajkumar P.

Afiliação

Haridhasapavalan KK; Laboratory for Stem Cell Engineering and Regenerative Medicine, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India.
Sundaravadivelu PK; Laboratory for Stem Cell Engineering and Regenerative Medicine, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India.
Bhattacharyya S; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India.
Ranjan SH; Laboratory for Stem Cell Engineering and Regenerative Medicine, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India.
Raina K; Laboratory for Stem Cell Engineering and Regenerative Medicine, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India.
Thummer RP; Laboratory for Stem Cell Engineering and Regenerative Medicine, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India. rthu@iitg.ac.in.

Bioprocess Biosyst Eng ; 44(6): 1131-1146, 2021 Jun.

Article em En | MEDLINE | ID: mdl-33559005

Assuntos

Escherichia coli; Fator de Transcrição GATA4; Linhagem Celular; Dicroísmo Circular; Escherichia coli/química; Escherichia coli/genética; Escherichia coli/metabolismo; Fator de Transcrição GATA4/biossíntese; Fator de Transcrição GATA4/química; Fator de Transcrição GATA4/genética; Fator de Transcrição GATA4/isolamento & purificação; Humanos; Estrutura Secundária de Proteína; Proteínas Recombinantes/biossíntese; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/isolamento & purificação

Palavras-chave

E. coli; GATA4; Protein expression and purification; Recombinant protein; Secondary structure

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Escherichia coli / Fator de Transcrição GATA4 Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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