A DNA-Encoded Chemical Library Based on Peptide Macrocycles.
Chemistry
; 27(24): 7160-7167, 2021 Apr 26.
Article
em En
| MEDLINE
| ID: mdl-33586277
ABSTRACT
The synthesis and characterization of a novel DNA-encoded library of macrocyclic peptide derivatives are described; the macrocycles are based on three sets of proteinogenic and non-proteinogenic amino acid building blocks and featuring the use of copper(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reaction for ring closure. The library (termed YO-DEL) which contains 1 254 838 compounds, was encoded with DNA in single-stranded format and was screened against target proteins of interest using affinity capture procedures and photocrosslinking. YO-DEL selections yielded specific binders against serum albumins, carbonic anhydrases and NKp46, a marker of activated Natural Killer cells.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Anidrases Carbônicas
/
Bibliotecas de Moléculas Pequenas
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article