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Conceptus metabolomic profiling reveals stage-specific phenotypes leading up to pregnancy recognition in cattle†.
Simintiras, Constantine A; Sánchez, José M; McDonald, Michael; O'Callaghan, Elena; Aburima, Ahmed A; Lonergan, Patrick.
Afiliação
  • Simintiras CA; School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.
  • Sánchez JM; School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.
  • McDonald M; School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.
  • O'Callaghan E; School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.
  • Aburima AA; Centre for Atherothrombotic and Metabolic Research, Hull York Medical School, Kingston-Upon-Hull, UK.
  • Lonergan P; School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.
Biol Reprod ; 104(5): 1022-1033, 2021 05 07.
Article em En | MEDLINE | ID: mdl-33590828
ABSTRACT
Reproductive efficiency in livestock is a major driver of sustainable food production. The poorly understood process of ruminant conceptus elongation (a) prerequisites maternal pregnancy recognition, (b) is essential to successful pregnancy establishment, and (c) coincides with a period of significant conceptus mortality. Conceptuses at five key developmental stages between Days 8-16 were recovered and cultured in vitro for 6 h prior to conditioned media analysis by untargeted ultrahigh-performance liquid chromatography tandem mass spectroscopy. This global temporal biochemical interrogation of the ex situ bovine conceptus unearths two antithetical stage-specific metabolic phenotypes during tubular (metabolically retentive) vs. filamentous (secretory) development. Moreover, the retentive conceptus phenotype on Day 14 coincides with an established period of elevated metabolic density in the uterine fluid of heifers with high systemic progesterone-a model of accelerated conceptus elongation. These data, combined, suggest a metabolic mechanism underpinning conceptus elongation, thereby enhancing our understanding of the biochemical reciprocity of maternal-conceptus communication, prior to maternal pregnancy recognition.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fenótipo / Prenhez / Bovinos / Embrião de Mamíferos / Metaboloma / Criação de Animais Domésticos Limite: Animals / Pregnancy Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fenótipo / Prenhez / Bovinos / Embrião de Mamíferos / Metaboloma / Criação de Animais Domésticos Limite: Animals / Pregnancy Idioma: En Ano de publicação: 2021 Tipo de documento: Article