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Identification and characterization of two novel ISCR1-associated genes dfrA42 and dfrA43 encoding trimethoprim resistant dihydrofolate reductases.
Li, Ling; Zhang, Mengge; Wang, Wenjia; Xia, Ruirui; Ma, Yanan; Wei, Xin; Wang, Xinhua; Sun, Xiaomin; Xie, Xiaohong; Xie, Shiling; Wang, Mingyu; Xu, Hai.
Afiliação
  • Li L; State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, Shandong, China.
  • Zhang M; State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, Shandong, China.
  • Wang W; State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, Shandong, China.
  • Xia R; State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, Shandong, China.
  • Ma Y; State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, Shandong, China.
  • Wei X; State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, Shandong, China.
  • Wang X; Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao, Shandong, China.
  • Sun X; Environmental Research Institute, Shandong University, Qingdao, Shandong, China.
  • Xie X; Shandong Shtars Medical Technology Co., Ltd.
  • Xie S; Shandong Shtars Medical Technology Co., Ltd.
  • Wang M; State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, Shandong, China. wangmingyu@sdu.edu.cn haixu@sdu.edu.cn.
  • Xu H; State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, Shandong, China. wangmingyu@sdu.edu.cn haixu@sdu.edu.cn.
Antimicrob Agents Chemother ; 95(5)2023 05 01.
Article em En | MEDLINE | ID: mdl-33593833
ABSTRACT
Two novel ISCR1-associated dfr genes, dfrA42 and dfrA43, were identified from trimethoprim (TMP)-resistant Proteus strains and were shown to confer high level TMP resistance (MIC ≥ 1024 mg/L) when cloned into Escherichia coli These genes were hosted by complex class 1 integrons suggesting their potentials for dissemination. Analysis of enzymatic parameters and TMP affinity were performed, suggesting that the mechanism of TMP resistance for these novel DHFRs is the reduction of binding with TMP.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Risk_factors_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Risk_factors_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article