Sleep Deprivation Interferes with JAK/STAT Signaling Pathway and Myogenesis in the Masseter Muscle of Rats.
Med Princ Pract
; 30(3): 253-261, 2021.
Article
em En
| MEDLINE
| ID: mdl-33601387
ABSTRACT
OBJECTIVES:
The aim of the study was to study the Janus kinase/tyrosine kinase-activated transduction factor (JAK/STAT) signaling pathway and myogenesis on the masseter muscle after sleep deprivation and to investigate the role of stress in this scenario. SUBJECTS ANDMETHODS:
A total of 18 male Wistar rats were divided into the following groups control (n = 6) animals were not submitted to any procedures, and paradoxical sleep deprivation and vehicle (PSD + V; n = 6) animals were subjected to PSD for 96 h and (PSD + MET; n = 6) animals were subjected to PSD for 96 h with administration of metyrapone. Paradoxical sleep deprivation was performed by the modified multiple platforms method. Histopathological analysis, histomorphometry, and immunohistochemistry were performed.RESULTS:
The results showed the presence of inflammatory infiltrate in the PSD + V and PSD + MET groups and atrophy. Histomorphometry showed that the cellular profile area decreased, while cellular density increased in both experimental groups. Expression of p-STAT 3, MyoD, and MyoG increased in the PSD + V group, while the PSD + MET group showed increased expression of IL-6 and p-STAT 3.CONCLUSION:
Our results suggest that sleep deprivation induces an inflammatory response and atrophy in the masseter muscle of rats.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Atrofia
/
Privação do Sono
/
Proteínas Tirosina Quinases
/
Atrofia Muscular
/
Desenvolvimento Muscular
/
Janus Quinases
/
Músculo Masseter
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article