Protocol for dissection and culture of murine dorsal root ganglia neurons to study neuropeptide release.
STAR Protoc
; 2(1): 100333, 2021 03 19.
Article
em En
| MEDLINE
| ID: mdl-33615276
ABSTRACT
In this protocol, we provide step-by-step instructions for dissection and culture of primary murine dorsal root ganglia (DRG), which provide an opportunity to study the functional properties of peripheral sensory neurons in vitro. Further, we describe the analysis of neuropeptide release by ELISA as a possible downstream application. In addition, isolated DRGs can be used directly for immunofluorescence, flow cytometry, RNA sequencing or proteomic approaches, electrophysiology, and calcium imaging. For complete details on the use and execution of this protocol, please refer to Perner et al. (2020).
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Receptoras Sensoriais
/
Neuropeptídeos
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Proteômica
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Gânglios Espinais
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article