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A polymer index-matched to water enables diverse applications in fluorescence microscopy.
Han, Xiaofei; Su, Yijun; White, Hamilton; O'Neill, Kate M; Morgan, Nicole Y; Christensen, Ryan; Potarazu, Deepika; Vishwasrao, Harshad D; Xu, Stephen; Sun, Yilun; Huang, Shar-Yin; Moyle, Mark W; Dai, Qionghai; Pommier, Yves; Giniger, Edward; Albrecht, Dirk R; Probst, Roland; Shroff, Hari.
Afiliação
  • Han X; Laboratory of High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA. suy4nih@gmail.com and Department of Automation, Tsinghua University, Beijing, 100084, China. hxf16@mails.tsinghua.edu.cn.
  • Su Y; Laboratory of High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA. suy4nih@gmail.com and Advanced Imaging and Microscopy Resource, National Institutes of Health, Bethesda, MD 20892, USA.
  • White H; Department of Biomedical Engineering, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA. hawhite@wpi.edu and Department of Neurobiology, University of Massachusetts Medical School, Worcester, MA 01655, USA.
  • O'Neill KM; National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA and Institute for Physical Science and Technology, University of Maryland College Park, College Park, MD 20742, USA.
  • Morgan NY; National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA.
  • Christensen R; Laboratory of High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA. suy4nih@gmail.com.
  • Potarazu D; Laboratory of High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA. suy4nih@gmail.com.
  • Vishwasrao HD; Advanced Imaging and Microscopy Resource, National Institutes of Health, Bethesda, MD 20892, USA.
  • Xu S; Laboratory of High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA. suy4nih@gmail.com.
  • Sun Y; Developmental Therapeutics Branch and Laboratory of Molecular Pharmacology, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, USA.
  • Huang SY; Developmental Therapeutics Branch and Laboratory of Molecular Pharmacology, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, USA.
  • Moyle MW; Department of Neuroscience and Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06536, USA.
  • Dai Q; Department of Automation, Tsinghua University, Beijing, 100084, China. hxf16@mails.tsinghua.edu.cn.
  • Pommier Y; Developmental Therapeutics Branch and Laboratory of Molecular Pharmacology, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, USA.
  • Giniger E; National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
  • Albrecht DR; Department of Biomedical Engineering, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA. hawhite@wpi.edu and Department of Biology and Biotechnology, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA.
  • Probst R; ACUITYnano, Innovation in Biomedical Imaging, North Bethesda, MD 20850, USA.
  • Shroff H; Laboratory of High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA. suy4nih@gmail.com and Advanced Imaging and Microscopy Resource, National Institutes of Health, Bethesda, MD 20892, USA and Marine Biolog
Lab Chip ; 21(8): 1549-1562, 2021 04 20.
Article em En | MEDLINE | ID: mdl-33629685
ABSTRACT
We demonstrate diffraction-limited and super-resolution imaging through thick layers (tens-hundreds of microns) of BIO-133, a biocompatible, UV-curable, commercially available polymer with a refractive index (RI) matched to water. We show that cells can be directly grown on BIO-133 substrates without the need for surface passivation and use this capability to perform extended time-lapse volumetric imaging of cellular dynamics 1) at isotropic resolution using dual-view light-sheet microscopy, and 2) at super-resolution using instant structured illumination microscopy. BIO-133 also enables immobilization of 1) Drosophila tissue, allowing us to track membrane puncta in pioneer neurons, and 2) Caenorhabditis elegans, which allows us to image and inspect fine neural structure and to track pan-neuronal calcium activity over hundreds of volumes. Finally, BIO-133 is compatible with other microfluidic materials, enabling optical and chemical perturbation of immobilized samples, as we demonstrate by performing drug and optogenetic stimulation on cells and C. elegans.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Água / Caenorhabditis elegans Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Água / Caenorhabditis elegans Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article