Protocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insects.
Sci Rep
; 11(1): 4717, 2021 02 25.
Article
em En
| MEDLINE
| ID: mdl-33633143
ABSTRACT
Modern morphological and structural studies are coming to a new level by incorporating the latest methods of three-dimensional electron microscopy (3D-EM). One of the key problems for the wide usage of these methods is posed by difficulties with sample preparation, since the methods work poorly with heterogeneous (consisting of tissues different in structure and in chemical composition) samples and require expensive equipment and usually much time. We have developed a simple protocol allows preparing heterogeneous biological samples suitable for 3D-EM in a laboratory that has a standard supply of equipment and reagents for electron microscopy. This protocol, combined with focused ion-beam scanning electron microscopy, makes it possible to study 3D ultrastructure of complex biological samples, e.g., whole insect heads, over their entire volume at the cellular and subcellular levels. The protocol provides new opportunities for many areas of study, including connectomics.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microscopia Eletrônica de Varredura
/
Microscopia Eletrônica de Transmissão
/
Insetos
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article