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Identification of chondroitin polymerizing factor (CHPF) as tumor promotor in cholangiocarcinoma through regulating cell proliferation, cell apoptosis and cell migration.
Duan, Xiaohui; Yang, Jianhui; Jiang, Bo; Duan, Wenbin; Wei, Rongguang; Zhang, Hui; Mao, Xianhai.
Afiliação
  • Duan X; Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, the First-affiliated Hospital of Hunan Normal University, Changsha, Hunan, China.
  • Yang J; Research Laboratory of Hepatobiliary Tumor,Hunan Provincial People's Hospital, the First-affiliated Hospital of Hunan Normal University, Changsha, Hunan, China.
  • Jiang B; Clinical Medical Research Center for Biliary Disease of Hunan Province, Changsha, China.
  • Duan W; Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, the First-affiliated Hospital of Hunan Normal University, Changsha, Hunan, China.
  • Wei R; Research Laboratory of Hepatobiliary Tumor,Hunan Provincial People's Hospital, the First-affiliated Hospital of Hunan Normal University, Changsha, Hunan, China.
  • Zhang H; Clinical Medical Research Center for Biliary Disease of Hunan Province, Changsha, China.
  • Mao X; Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, the First-affiliated Hospital of Hunan Normal University, Changsha, Hunan, China.
Cell Cycle ; 20(5-6): 591-602, 2021.
Article em En | MEDLINE | ID: mdl-33651657
Cholangiocarcinoma (CCA) is a variety of biliary epithelial tumors involving intrahepatic, perihilar and distal bile duct. It is the most common malignant bile duct tumor in the liver and the second most common primary liver cancer, whose molecular mechanism not fully understood. Specifically, the relationship between CCA and chondroitin polymerizing factor (CHPF) is still not clear. In this study, detection of clinical specimens was performed to preliminarily study the role of CHPF in CCA. CCA cells with CHPF knockdown were constructed for in vitro study, which was also used in the construction of mice xenograft model for investigating the role of CHPF in the development of CCA. The results demonstrated that CHPF was significantly upregulated in CCA tissues compared with normal tissues. High expression of CHPF was correlated with more advanced tumor grade. Moreover, knockdown of CHPF significantly inhibited cell proliferation, cell migration, promoted cell apoptosis and arrest cell cycle in G2 phase in vitro, as well as suppressed tumor growth in vivo. In conclusion, CHPF was identified as a tumor promotor in the development and metastasis of CCA, which may provide a novel therapeutic target for the targeted therapy against CCA.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias dos Ductos Biliares / Carcinógenos / Movimento Celular / N-Acetilgalactosaminiltransferases / Colangiocarcinoma / Proliferação de Células Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias dos Ductos Biliares / Carcinógenos / Movimento Celular / N-Acetilgalactosaminiltransferases / Colangiocarcinoma / Proliferação de Células Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article