PIONEER: Pipeline for Generating High-Quality Spectral Libraries for DIA-MS Data.
Curr Protoc
; 1(3): e69, 2021 Mar.
Article
em En
| MEDLINE
| ID: mdl-33656278
ABSTRACT
Data-independent-acquisition mass spectrometry (DIA-MS) is a state-of-the-art proteomic technique for high-throughput identification and quantification of peptides and proteins. Interpretation of DIA-MS data relies on the use of a spectral library, which is optimally created from data acquired from the same samples in data-dependent acquisition (DDA) mode. As DIA-MS quantification relies on the spectral libraries, having a high-quality, non-redundant, and comprehensive spectral library is essential. This article describes the major steps for creating a high-quality spectral library using a combination of multiple complementary search engines. We discuss appropriate strategies to control the false discovery rate for the final spectral library as a result of merging multiple searches. © 2021 The Authors Current Protocols © 2021 Wiley Periodicals LLC. Basic Protocol 1 Searching DDA-MS files with multiple search engines Basic Protocol 2 Merging results from multiple search engines Basic Protocol 3 Creating spectral libraries from merged results Alternate Protocol Using CLI for automating tasks Support Protocol Creating concatenated FASTA files.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Proteômica
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article