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Transmission of SARS-CoV-2 in northern Ghana: insights from whole-genome sequencing.
Sylverken, Augustina Angelina; El-Duah, Philip; Owusu, Michael; Schneider, Julia; Yeboah, Richmond; Ayisi-Boateng, Nana Kwame; Gorman, Richmond; Adu, Eric; Kwarteng, Alexander; Frimpong, Michael; Binger, Tabea; Aryeetey, Sherihane; Asamoah, Jesse Addo; Amoako, Yaw Ampem; Amuasi, John Humphrey; Beheim-Schwarzbach, Jörn; Owusu-Dabo, Ellis; Adu-Sarkodie, Yaw; Obiri-Danso, Kwasi; Corman, Victor Max; Drosten, Christian; Phillips, Richard.
Afiliação
  • Sylverken AA; Kumasi Centre for Collaborative Research in Tropical Medicine, PMB, UPO, 00233, Kumasi, Ghana. asylverken@knust.edu.gh.
  • El-Duah P; Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana. asylverken@knust.edu.gh.
  • Owusu M; Kumasi Centre for Collaborative Research in Tropical Medicine, PMB, UPO, 00233, Kumasi, Ghana.
  • Schneider J; Institute of Virology, Charite, Universitätsmedizin Berlin, Berlin, Germany.
  • Yeboah R; Kumasi Centre for Collaborative Research in Tropical Medicine, PMB, UPO, 00233, Kumasi, Ghana.
  • Ayisi-Boateng NK; Department of Medical Diagnostics, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
  • Gorman R; Institute of Virology, Charite, Universitätsmedizin Berlin, Berlin, Germany.
  • Adu E; Kumasi Centre for Collaborative Research in Tropical Medicine, PMB, UPO, 00233, Kumasi, Ghana.
  • Kwarteng A; Department of Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
  • Frimpong M; Kumasi Centre for Collaborative Research in Tropical Medicine, PMB, UPO, 00233, Kumasi, Ghana.
  • Binger T; Kumasi Centre for Collaborative Research in Tropical Medicine, PMB, UPO, 00233, Kumasi, Ghana.
  • Aryeetey S; Kumasi Centre for Collaborative Research in Tropical Medicine, PMB, UPO, 00233, Kumasi, Ghana.
  • Asamoah JA; Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
  • Amoako YA; Kumasi Centre for Collaborative Research in Tropical Medicine, PMB, UPO, 00233, Kumasi, Ghana.
  • Amuasi JH; Department of Molecular Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
  • Beheim-Schwarzbach J; Kumasi Centre for Collaborative Research in Tropical Medicine, PMB, UPO, 00233, Kumasi, Ghana.
  • Owusu-Dabo E; Kumasi Centre for Collaborative Research in Tropical Medicine, PMB, UPO, 00233, Kumasi, Ghana.
  • Adu-Sarkodie Y; Kumasi Centre for Collaborative Research in Tropical Medicine, PMB, UPO, 00233, Kumasi, Ghana.
  • Obiri-Danso K; Department of Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
  • Corman VM; Kumasi Centre for Collaborative Research in Tropical Medicine, PMB, UPO, 00233, Kumasi, Ghana.
  • Drosten C; Department of Global and International Health, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
  • Phillips R; Institute of Virology, Charite, Universitätsmedizin Berlin, Berlin, Germany.
Arch Virol ; 166(5): 1385-1393, 2021 May.
Article em En | MEDLINE | ID: mdl-33723631
Following the detection of the first imported case of COVID-19 in the northern sector of Ghana, we molecularly characterized and phylogenetically analysed sequences, including three complete genome sequences, of severe acute respiratory syndrome coronavirus 2 obtained from nine patients in Ghana. We performed high-throughput sequencing on nine samples that were found to have a high concentration of viral RNA. We also assessed the potential impact that long-distance transport of samples to testing centres may have on sequencing results. Here, two samples that were similar in terms of viral RNA concentration but were transported from sites that are over 400 km apart were analyzed. All sequences were compared to previous sequences from Ghana and representative sequences from regions where our patients had previously travelled. Three complete genome sequences and another nearly complete genome sequence with 95.6% coverage were obtained. Sequences with coverage in excess of 80% were found to belong to three lineages, namely A, B.1 and B.2. Our sequences clustered in two different clades, with the majority falling within a clade composed of sequences from sub-Saharan Africa. Less RNA fragmentation was seen in sample KATH23, which was collected 9 km from the testing site, than in sample TTH6, which was collected and transported over a distance of 400 km to the testing site. The clustering of several sequences from sub-Saharan Africa suggests regional circulation of the viruses in the subregion. Importantly, there may be a need to decentralize testing sites and build more capacity across Africa to boost the sequencing output of the subregion.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sequenciamento Completo do Genoma / SARS-CoV-2 / COVID-19 Tipo de estudo: Clinical_trials Limite: Female / Humans / Male País/Região como assunto: Africa Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sequenciamento Completo do Genoma / SARS-CoV-2 / COVID-19 Tipo de estudo: Clinical_trials Limite: Female / Humans / Male País/Região como assunto: Africa Idioma: En Ano de publicação: 2021 Tipo de documento: Article