Application of error-prone PCR to functionally probe the morbillivirus Haemagglutinin protein.
J Gen Virol
; 102(4)2021 04.
Article
em En
| MEDLINE
| ID: mdl-33739251
ABSTRACT
The enveloped morbilliviruses utilise conserved proteinaceous receptors to enter host cells SLAMF1 or Nectin-4. Receptor binding is initiated by the viral attachment protein Haemagglutinin (H), with the viral Fusion protein (F) driving membrane fusion. Crystal structures of the prototypic morbillivirus measles virus H with either SLAMF1 or Nectin-4 are available and have served as the basis for improved understanding of this interaction. However, whether these interactions remain conserved throughout the morbillivirus genus requires further characterisation. Using a random mutagenesis approach, based on error-prone PCR, we targeted the putative receptor binding site for SLAMF1 interaction on peste des petits ruminants virus (PPRV) H, identifying mutations that inhibited virus-induced cell-cell fusion. These data, combined with structural modelling of the PPRV H and ovine SLAMF1 interaction, indicate this region is functionally conserved across all morbilliviruses. Error-prone PCR provides a powerful tool for functionally characterising functional domains within viral proteins.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Moléculas de Adesão Celular
/
Proteínas Virais de Fusão
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Reação em Cadeia da Polimerase
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Vírus da Peste dos Pequenos Ruminantes
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Membro 1 da Família de Moléculas de Sinalização da Ativação Linfocitária
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Hemaglutininas Virais
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article