Optobiochemistry: Genetically Encoded Control of Protein Activity by Light.
Annu Rev Biochem
; 90: 475-501, 2021 06 20.
Article
em En
| MEDLINE
| ID: mdl-33781076
Optobiochemical control of protein activities allows the investigation of protein functions in living cells with high spatiotemporal resolution. Over the last two decades, numerous natural photosensory domains have been characterized and synthetic domains engineered and assembled into photoregulatory systems to control protein function with light. Here, we review the field of optobiochemistry, categorizing photosensory domains by chromophore, describing photoregulatory systems by mechanism of action, and discussing protein classes frequently investigated using optical methods. We also present examples of how spatial or temporal control of proteins in living cells has provided new insights not possible with traditional biochemical or cell biological techniques.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bioquímica
/
Proteínas
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article