In Vitro Stimulation of Whole Milk Specimens: A Field-Friendly Method to Assess Milk Immune Activity.
J Hum Lact
; 37(4): 736-745, 2021 11.
Article
em En
| MEDLINE
| ID: mdl-33788640
ABSTRACT
BACKGROUND:
The immune system of milk protects against infections and guides immune system development. A system-level understanding of milk immune activity is critical for research into infant infectious disease risk and lifelong health. RESEARCHAIM:
To describe a protocol to characterize immune activity in human milk via in vitro stimulation for use in population-based (rather than clinical) research.METHODS:
This study proceeded in two phases, each with a cross-sectional design. Human milk specimens were incubated for 24 hr at 37 °C in mammalian cell culture medium with stimuli (e.g., Salmonella enterica) in a CO2-enriched environment. Immune responses to stimuli were characterized as the change in cytokine [stimulated]/[baseline]. Predictors of cytokine responses were evaluated with generalized linear models.RESULTS:
Patterns were detectable across mother-child dyads Interleukin-6 responses to stimuli were generally positively associated with child age and with maternal autoimmune disease.CONCLUSIONS:
Our method allows characterization of pro-inflammatory milk immune activity in vitro in population-based (rather than clinical) research settings. In vitro activity has a system-level interpretation and is likely to be of broad utility in global health research in settings with high infectious disease risk, where understanding the immune system of milk is critical to understanding maternal and child health.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aleitamento Materno
/
Leite Humano
Tipo de estudo:
Guideline
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Observational_studies
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Prevalence_studies
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Prognostic_studies
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Risk_factors_studies
Limite:
Animals
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Female
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Humans
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Infant
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article