Non-Coding RNA Silencing in Mammalian Cells by Antisense LNA GapmeRs Transfection.
Methods Mol Biol
; 2300: 31-37, 2021.
Article
em En
| MEDLINE
| ID: mdl-33792869
ABSTRACT
The assessment of non-coding RNAs (ncRNAs) functions highly relies on loss of function studies. However, due to their exclusive or partial nuclear localization, many small and long ncRNAs are not efficiently silenced by RNA interference. Antisense LNA GapmeRs constitute a good alternative to RNAi. They allow an effective knockdown of ncRNAs with sizes greater than 80 nucleotides, regardless of their cellular localization. This chapter focuses on the silencing of two different nuclear ncRNAs (ANRIL and SATIII RNAs) in mammalian cells using antisense LNA GapmeRs with two different transfection methods:
calcium phosphate-mediated transfection and LipofectamineTM 2000.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transfecção
/
Oligonucleotídeos Antissenso
/
RNA Longo não Codificante
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article